Tubulin, alpha (ATU1)

BLASTp Hit to Protein of Known or Suspected Function:
Organism: Tetrahymena thermophila
GI Number: 730899
E-value:  0

Protein and Coding Sequences:

TGD ID Number: 73.m00202

Evidence: TIGR Preliminary Gene Prediction 08/2004: predicted gene structure is partially supported by ESTs.

Coding Sequence:

ATGAGAGAAGTTATTTCAATTCACGTCGGTCAAGGTGGTATCCAAGTCGGTAACGCCTGT
TGGGAATTATTCTGTCTTGAACACGGTATCTAGCCTGATGGTCAAATGCCCTCTGATAAG
ACTATCGGTGGTGGTGATGATGCTTTCAACACCTTCTTCTCTGAAACTGGTGCCGGTAAG
CACGTCCCCAGAGCTGTCTTCTTAGATCTTGAACCCACTGTCATCGATGAAGTCAGAACT
GGTACCTACAGACAATTATTCCACCCTGAATAATTGATCTCCGGTAAGGAAGATGCCGCT
AACAACTTCGCCAGAGGTCACTACACCATTGGTAAGGAAATCGTCGATCTCTGCTTAGAC
AGAATCAGAAAGTTGGCTGATAACTGTACTGGTCTCCAAGGTTTCTTAGTCTTCAACTCC
GTCGGTGGTGGTACCGGTTCCGGTTTGGGTTCTCTCCTTTTGGAAAGACTCTCCGTCGAT
TACGGTAAGAAATCCAAGTTGGGCTTCACCATCTATCCCTCCCCCTAAGTCTCAACCGCC
GTCGTTGAACCCTACAACTCCATTCTTTCTACTCACTCTTTGTTAGAACACACTGATGTC
GCCGTTATGCTCGATAACGAAGCCATCTATGATATCTGCAGAAGAAACCTCGATATCGAA
AGACCCACCTACACTAACTTGAACAGACTTATTGCTTAAGTTATCTCCTCTTTGACTGCC
TCCCTCAGATTCGATGGTGCCCTTAACGTCGATATCACTGAATTCTAAACTAACTTGGTC
CCCTATCCTAGAATTCACTTTATGCTTTCATCTTATGCTCCTATCATCTCCGCTGAAAAG
GCTTATCACGAATAACTCTCCGTTGCTGAAATCACCAACTCTGCCTTCGAACCCGCTAAC
ATGATGGCTAAGTGTGATCCCAGACACGGTAAGTACATGGCTTGCTCTATGATGTACAGA
GGTGATGTCGTCCCCAAGGATGTCAACGCTTCTATTGCCACCATCAAGACCAAGAGAACC
ATCCAATTCGTCGACTGGTGCCCCACTGGTTTCAAGGTCGGTATTAACTATCAACCCCCC
ACCGTCGTTCCCGGTGGTGATCTTGCTAAGGTCATGAGAGCTGTCTGTATGATTTCCAAC
TCTACTGCCATCGCTGAAGTCTTCTCCAGACTTGACCACAAGTTCGATCTTATGTACGCC
AAGAGAGCCTTCGTCCACTGGTACGTCGGTGAAGGTATGGAAGAAGGTGAATTCTCCGAA
GCCAGAGAAGATCTTGCCGCCCTCGAAAAGGATTACGAAGAAGTCGGTATTGAAACTGCT
GAAGGTGAAGGTGAAGAAGAAGGTTACTGA

Protein Sequence:

MREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGK
HVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD
RIRKLADNCTGLQGFLVFNSVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTA
VVEPYNSILSTHSLLEHTDVAVMLDNEAIYDICRRNLDIERPTYTNLNRLIAQVISSLTA
SLRFDGALNVDITEFQTNLVPYPRIHFMLSSYAPIISAEKAYHEQLSVAEITNSAFEPAN
MMAKCDPRHGKYMACSMMYRGDVVPKDVNASIATIKTKRTIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVMRAVCMISNSTAIAEVFSRLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDLAALEKDYEEVGIETAEGEGEEEGY*

Peptides Identified in Mass Spectrometry Analysis: 4
NLDIERPTYTNLNR
AFVHWYVGEGMEEGEFSEAR
AVFLDLEPTVIDEVR
AYHEQLSVAEITNSAFEPANMMAK

Number of Independent Analyses from which Peptide Data was Obtained: 2

Relevant Tetrahymena References:

McGrath, K.E., et al. 1994. Regulation and evolution of the single alpha-tubulin gene of the ciliate Tetrahymena thermophila. Cell Motil. Cytoskeleton. 27(3): 272-283.

Presence in Phagosome Proteomes of Other Organisms:

Mus musculus (Garin, J., et al. 2001. The Phagosome Proteome: Insight Into Phagosome Functions. J Cell Biol. 152:165-180.)