Alpha-Amylase

BLASTp Hit to Protein of Known or Suspected Function:
Organism: Dictyostelium discoideum
GI Number: 60468532
E-value: 1e-115

Protein and Coding Sequences:

TGD ID Number: 44.m00246

Evidence: TIGR Preliminary Gene Prediction 08/2004: predicted gene structure is partially supported by ESTs.

Coding Sequence:

ATGAGCAAACTCTAATTTGTATTAATTGCTGCACTTCTTCTTGTTGCAGTCTCTGCTAAA
ACCGCTTAGGAATGGAAGACACGTAACATTTATCAAATCATCACTGATAGATTCGCTCTT
GGTGATGGATCTAAGCCATACTGTGATTTAAATAAATAACCCTACTATTGTGGTGGTAAC
TTCAAGGGTATTGAGAACAATCTTGACTACATTCAAGGTATGGGTTTCAATGCTATTTGG
ATCTCTCCTGTTCCTGATAACTACCCCAACTAATTCCATGGTTATGCTGCCAAGAACATG
ACTGAAATAAACAGCCATTTTGGTGGTGCTGATGGTCTTAAATCTCTTATTGATGCATGC
CACAAGAGAGATATCTGGGTTATGATTGATGTTGTTGCTAACCACATGGGTAACACCGAT
TAAAACTACTCTGAAAATATTCCCTTCAACTCTCCTGATCACTATCACTCTTACTGTATT
ATTTCTGATTCAGATTTCGCAACTAAGAATATGTACAACATTCAACACTGCAGACTTGCT
GGGCTTGCCGACCTTAACTAAGAAAATTCTTATGTTAGCAACTATCTTGTTAACTGGATT
AAGGATCTTGTTTAAACCTACAACGTTGATGGTATTAGAATAGACACTGTCCCTGAAGTA
CACCCTGACTTCTGGAAATAATATACTACCTCTGCTGGTGTTTTTGCTACTGGTGAAGTT
TTTGATGGTGATTTTGGATATGTTGCCAGCTACATTCCCTCTGTTGGCTCAGTCTTGAAC
TATCCTTGGTACTTTAACATGAGAGATATCTTCGTTAACCAAAAGGATATGTGGGGAGTT
AGAACCTATTACTAAAATTGGGCTAATTAACACGCTGATCTTTCTATCCTTACTCCTTTC
GTTGATAATCACGATAATCCTCGTTTCTTATCTGATCAAGTCTTCAATAATGGTAAAGAC
AGAAACACTCGTATTCAACTCTTAAAGGGATACACAACTTTCACTCTTACATCTATTGGT
ATTCCTATTCTTTACTACGGTACTGAATAATACTTCTCTGGTAATACTGATCCTTATGAC
CGTGAACCTTTGTGGAACAGCTTAAACACTAACTCTGATATGTACAAATATGTCCAATCT
ATTTTATATGCTAAGAACATCACTAATGCTGGATTATAAGGATAAACTGAAGCTTATTGT
GACCAAGATTTATATGCTTTCTTCAGAGGTAAATTGTTCGTTGGTGTCACTTCTAAGTTT
AATTAAGTTACAAGACAAATTGTTACTCATCCCTTCTCAAATGGTGAAGTTATTTGCAAT
ATTTTCTATCCTACTTAAGATTGCCTTACAATTACTAATAACTCCTTCAACCTTGTTCTT
ATTGGTGGTGAAGCTAAGATCTTTGTTCCTAAATCTCTCCTCTAAAAATCAAGTTAATTT
TTGAGATCTGCTTCTAGTTGA

Protein Sequence:

MSKLQFVLIAALLLVAVSAKTAQEWKTRNIYQIITDRFALGDGSKPYCDLNKQPYYCGGN
FKGIENNLDYIQGMGFNAIWISPVPDNYPNQFHGYAAKNMTEINSHFGGADGLKSLIDAC
HKRDIWVMIDVVANHMGNTDQNYSENIPFNSPDHYHSYCIISDSDFATKNMYNIQHCRLA
GLADLNQENSYVSNYLVNWIKDLVQTYNVDGIRIDTVPEVHPDFWKQYTTSAGVFATGEV
FDGDFGYVASYIPSVGSVLNYPWYFNMRDIFVNQKDMWGVRTYYQNWANQHADLSILTPF
VDNHDNPRFLSDQVFNNGKDRNTRIQLLKGYTTFTLTSIGIPILYYGTEQYFSGNTDPYD
REPLWNSLNTNSDMYKYVQSILYAKNITNAGLQGQTEAYCDQDLYAFFRGKLFVGVTSKF
NQVTRQIVTHPFSNGEVICNIFYPTQDCLTITNNSFNLVLIGGEAKIFVPKSLLQKSSQF
LRSASS*

Peptides Identified in Mass Spectrometry Analysis: 1
DLVQTYNVDGIR

Number of Independent Analyses from which Peptide Data was Obtained: 1

Relevant Tetrahymena References:

 

Presence in Phagosome Proteomes of Other Organisms:

Entamoeba histolytica (Okada, M., et al. 2005. Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica. Euk Cell. 4:827-831.)