Tetrahymena Phagosome Proteome
This page presents a listing of proteins identified in an analysis of the Tetrahymena thermophila phagosome proteome by mass spectrometry (Jacobs, M.E., et al., 2006. Eukaryot. Cell 5:1990-2000) that has been carried out by the Klobutcher lab in collaboration with Centre for Research in Mass Spectrometry at York University that is headed by Dr. K. W. Michael Siu. The proteins are grouped into: 1. Strong Candidates with Homologs of Known Function in Other Organisms; 2. Strong Candidates with Unknown Function; 3. Less Well Supported Candidates (single peptide identified in a single analysis, but phagosome-associated in other organisms); and 4. Likely Contaminants. Each entry is linked to an information page that provides the amino acid sequence of the protein and coding DNA sequence. The protein/gene sequence information is based primarily on the preliminary annotation of the Tetrahymena macronuclear genome sequence (Eisen, J. A., et al., 2006. PLoS Biol. 4: e286), but we have refined some of the gene predictions based on either cDNA/EST data or through comparisons to homologous proteins in other organisms. Each entry also includes the sequence(s) of the peptides identified in the MS analyses, information regarding whether the protein has been identified in the phagosome proteome of other organisms or if it is listed as a component of phagosomes or lytic vacuoles in the GO database, and relevant Tetrahymena literature. Information on mutant phenotypes and sub-cellular location is available in the literature for some proteins but it has not yet been entered on this web site.
A. Strong Candidates with Homologs of Known Function in Other Organisms:
14-3-3-like protein (14-3-3 protein)
Acetyl-CoA Acyltransferase [3-ketoacyl-CoA thiolase]
Acid Alpha-glucosidase
Acid Phosphatase
Acid Phosphatase
ADP-ribosylation Factor
ATP-binding Cassette Transporter
ATP-binding Cassette (ABC) Transporter, sub-family C
Bactericidal/Permeability-increasing Protein
Calcium ATPase (TPA8)
Carbonic Anhydrase-like Protein
Cathepsin B
Cathepsin B
Cathepsin L/Tetrain
Cathepsin L-like Protein
Cathepsin L
Chitinase-related Protein
Cytochrome b5-like, Heme/Steroid-binding Domain
Cytochrome P450 Monooxygenase-related Protein
Elongation Factor 1-alpha
Elongation Factor 1-beta
Endoglycoceramidase (cellulose domain)
Alpha-Galactosidase/Hydrolase
GTP-binding Protein (RHD3-family)
GTP-binding Protein (Small; Sar 1-family)
Heat Shock Protein Hsp-70
Heat Shock Protein Hsp-70
Histone H2B2
Histone H4 (HHF2)
Lysosomal Acid Lipase/Gastric Lipase
Lysosomal Acid Phosphatase
Lysosomal Phospholipase A1/Cathepsin L
Methyltransferase-related Protein
Na+/K+-transporting ATPase, alpha subunit
Niemann-Pick C1
Palmitoyl-Protein Thioesterase
Peroxisomal Biogenesis Factor 11A-related Protein
Peroxisomal Multifunctional Enzyme
Phagosome Protein 1, Tetrahymena, (Php1p/P28p)
p28-Like Protein
Phospholipid Scramblase
Prolyl-4-hydroxylase (Thioredoxin domains)
Protein Disulfide-isomerase-related Protein
Protein Disulfide Isomerase
Rab1 Small GTP-binding Protein
Rab7 GTPase
Rab13 GTPase
Reticulocyte Binding-like Protein 4
Secreted Alpha Beta Hydrolase
Sequestosome 1
SerH3 Cell Surface Immobilization Antigen
Squalene-hopene Cyclase/Terpenesynthase
Surface Protein Type 51B-related Protein, Paramecium
Synaptobrevin/Longin/VAMP-related Protein
Tubulin, alpha (ATU1)
Tubulin, beta (BTU1)
Vacuolar ATPase, subunit A
Vacuolar ATPase, subunit a
Vacuolar ATPase, subunit d (C/AC39)
Vacuolar H+-translocating Inorganic Pyrophosphatase
Vps13 (Vacuolar Protein Sorting)/Chorein
B. Strong Candidates with Unknown Function:Tpp1p
Tpp2p
Tpp3p
Tpp5p
Tpp7p
Tpp9p
Tpp11p
Tpp12p
Tpp16p
Tpp19p
Tpp20p
Tpp21p
C. Less Well Supported Candidates (single peptide identified in a single analysis, but phagosome-associated in other organisms)
Actin Binding Protein
Actin Binding Protein
Actin Binding Protein
Alpha-Amylase
Cathepsin L Cysteine Protease
Cyclophilin
Cysteine Proteinase
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPC1)
Heat Shock Protein Hsp-90
Lipase
Alpha-Mannosidase
Protein Disulfide Isomerase-related Protein
Sialidase (Neuraminidase)
Ubiquitin
Vacuolar ATPase, subunit B
D. Likely Contaminants:
1. Proteins Associated with ExocytosisC-terminal Crystallin Fold Containing Protein 3p (CCF3)
Granule Lattice Protein 1
Granule Lattice Protein 1 (Grl1)
Granule Lattice Protein 3 (Grl3p)
Granule Lattice Protein 4 (Grl4p)
Granule Lattice Protein 5 (Grl5p)
Ndc1 protein (Grl8p)
Granule-biosynthesis Induced Protease (Gip1p)
Granule Tip Protein (Grt1p)
1. Proteins Associated with Exocytosis